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2 ) in control animals angiogenic response with all tested parameters (number of VEGF, CD34 and FVIII positive elements (data obtained on hematoxylin-eosin stain were equal to FVIII presentation and are not presented)) consistently reached its peak at day 7

The broccoli heads were withdrawn from the submergence solution after 24 h and the cut end of the stalk was blot dried

The pooled fractions (approximate 6 ml) were concentrated (approximate 0.6 ml) (centrifugal tube: Amicon Ultra-4 centrifugal filter unit with 10 kDa cutoff, from Millipore) and followed by 2D crystallization trials

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